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Neomycin, polymyxin B, and gramicidin is a mix antibiotic medicine used to take care of infections of the eye.The adenylation domain of GrsA synthetase (PheA protein) made up of the main 600 amino acids of GrsA was expressed recombinantly in E. coli M15 cells using the pQE60 vector. Protein purification was executed by way of Ni2+ affinity chromato